Table 3. Isolation methods for bovine muscle satellite cells published in 2023

Muscles	Enzymes	Centrifugation conditions	Pre-plating	References
Longissimus	Pronase	500×g, 10 min	N/A	Kim and Kim, 2023
Biceps femoris	Pronase	300×g, 5 min; 1,200×g, 15 min	N/A	Kim et al., 2023a
Longissimus	Pronase	500×g, 10 min	N/A	Kim et al., 2023b
Longissimus thoracis	Collagenase II, Dispase II	N/A	3 h+3 h	Lee et al., 2023b
Semitendinosus	Collagenase	N/A	N/A	Messmer et al., 2023
Top round	Collagenase mix	800×g, 5 min	N/A	Park et al., 2023
Semimembranosus	Collagenase, trypsin	100×g, 5 s; 1,000×g, 10 min	N/A	Skrivergaard et al., 2023
Semitendinosus	Collagenase II	N/A	N/A	Stout et al., 2023
Longissimus thoracis	Collagenase, trypsin	N/A	1 h	Tzimorotas et al., 2023
Longissimus lumbrorum	Collagenase D	76×g, 5 min	1 h	Uyen et al., 2023
Hind limb	Collagenase II, trypsin	N/A	N/A	Zhang et al., 2023
Longissimus dorsi	Collagenase II	500×g, 10 min	1 h	Zygmunt et al., 2023

N/A, not applicable.