Table 3. Application of lipidomics to assess food from farm animals

Sample Technology Lipid References
Meat
 Pork (Luchuan and Duroc pigs) UPLC-ESI-MS/MS 605 lipids, 23 categories, 211 TG, 95 PC, 66 PE, 46 DG, and 37 Car, Luchuan pigs have higher IMF, including more TG and DG (Zhang et al., 2021b)
 Pork (Tibetan, Jilin and Sanmenxia black pigs) UPLC-ESI-MS/MS 1,180 lipid species, Tibetan pork showed higher 14 GL, 8 GP, 2 SL, 2 FA, and 2 PR, Jilin pork showed higher 4 GL, 4 GP, 3 ST, 3 PK, 2 FA, and 2 PR (Mi et al., 2019a)
 Pork (Beijing Heiliu and Laiwu Chinese black, Duroc) UPLC-Q-Exactive Orbitrap/MS 757 lipids, black pork had more TG and less LPC, 55 potential markers (Li et al., 2021a)
 Pork (slaughter weight) UPLC-Q-Exactive Orbitrap/MS 592 lipids, 19 lipid classes, IMF and TG increased, PC and PE decreased with increasing slaughter weight (Li et al., 2021b)
 Black-boned silky fowl (different ages and genders) UPLC-ESI-Q-TOF/MS 1,127 lipids, 8 categories, GL and GP, 47 potential markers (Mi et al., 2018)
 Donkey meat (different parts) LC-MS 1,143 lipids and 14 subclasses, 73 differential lipids (Li et al., 2021c; Li et al., 2021d)
 Beef (different origins) LC-ESI-Q-TOF/MS Forty potential markers: GL, FA, PIs, PEs, LPEs, LPCs, Cer and SMs (Wang et al., 2021)
 Sheep meat (cold chain storage) UHPLC-Q-Orbitrap MS/MS Forty-eight changed lipids: 8 FAC, 23 PCs, 3 LPCs, 13 PEs, and 1 LPE (Jia et al., 2021)
Egg
 Chicken egg UPLC-Q-TOF MS/MS 1,633 lipid species, 43.78% GP, 25.66% GL, 16.66% FA, 6.86% SP, 4.10% ST, 1.53% PK, 1.10% PR, and 0.31% SL (Mi et al., 2019b)
 Egg yolks (duck, hen, and quail) UPLC-Q-TOF MS 57 molecular species of phospholipids (Ali et al., 2017)
 Chicken egg yolks High-resolution MS Glycerophosphocholines and glycerophosphoethanolamines (Wood et al., 2021)
 Chicken egg yolks LC-ESI-Q-TOF MS 618 lipid species, Tibetan and docosahexaenoic acid egg showed higher phospholipid and lower TG (Xie et al., 2020)
 Chicken egg (male and female ) LC-ESI-Q-TOF MS 900 lipid species, sex discrimination: acylcarnitine, TG (12:0/22:1/22:3), hemolytic serine (16:0/0:0), Cer (d18:0/18:0), cholesterol (16:1), and PC (18:3/20:4) (Xiang, 2019)
 Chicken egg (different diets and environments) HILIC-LC-MS/MS or UPLC-Q-TOF MS GL and GP (Campos et al., 2016; Wu et al., 2020)
Milk
 Donkey and cow milk (different lactation stages) UHPLC-Q-TOF MS 335 lipids, 13 subclasses, including CL, PA, PC, PE, PG, PI, PS, Cer, SMs, HexCer, Hex2Cers, DG, and TG (Li et al., 2020b; Li et al., 2020a)
 Goat milk (different geographical origins and lactation stages) UPLC-Q-Exactive Orbitrap MS 56 lipid species, 14 subclasses, including 5 CL, 45 Cer, 17 LPCs, 4 LPEs, 36 PCs, 80 PEs, 9 PGs, 22 PIs, 2 acyl carnitine species, 55 HexCer, 56 SMs, 17 FA, 15 DG, and 416 TG (Liu et al., 2020)
 Human, bovine, and caprine milk UHPLC-Q-TOF MS 13 lipid classes, including TG, DG, SM, PC, Cer, HexCer, Hex2Cer, PE, PG, PS, PI, PA, and CL (Wang et al., 2020)
 Donkey, human, and cow milk UHPLC-Q-Exactive Orbitrap MS 11 subclasses and 475 lipids, including 380 TG, 13 DG, 67 GP, 14 SP, and 1 wax exter (Zhang et al., 2021a)
 Bovine milk with vegetable oils or fats Matrix-assisted laser desorption/ionization-Q-TOF MS 20 TG classes with high speed and reliability (Garcia et al., 2012)
 Goat, soy, and bovine milk UPLC-Q-Exactive Orbitrap MS Goat milk was rich in medium-chain TG, USFA, and n-6 and n-3 FA, soy milk was rich in PC, PE, PS, and PG, while bovine milk was rich in TG, DG, and Cer, fourteen lipids potential markers (Li et al., 2017)
 Cow milk (heat stress of cow) UHPLC-Q-TOF MS Heat stress increased long-chain fatty acids; moreover, reduced PE, PS, PC, LPC and glucoceramide, potential markers: LPC (Liu et al., 2017)
UPLC, ultrahigh performance liquid chromatography; ESI, electrospray ionization; MS, mass spectrometry; IMF, intramuscular fat; LPCs, lysophosphatidylcholines; Q-TOF, quadrupole-time-of-flight; LC-MS, liquid chromatography-MS; LPEs, lysophosphatidylethanolamines; FAC, FA carnitines.